ISO 17721-1 pdf free download

ISO 17721-1-2021 pdf free download.Quantitative determination of antibacterial activity of ceramic tile surfaces一Test methods一 Part 1: Ceramic tile surfaces with incorporated antibacterial agents.
Other culture media may be selected based on the requirements for the bacteria selected for the testing.
Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 °C – 10 °C for up to one month.
4.2.3 1/500 nutrient broth
For 1 000 ml of purified water, take 3,0 g of meat extract, 10,0 g of peptone and 5,0 g of sodium chloride, put them in a flask and dissolve them thoroughly. Dilute this medium 500 times using distilled or deionized water, and set the pH to (7,0 ± 0,2) using hydrochloric acid solution or sodium hydroxide solution. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if 1/500 nutrient broth is not used immediately, store it at 5 °C to 10 °C.
Other culture media may be selected based on the requirements for the bacteria selected for the testing. Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 °C – 10°C for up to one month.
4.2.4 Nutrient agar
For 1 000 ml of purified water, take 3,0 g of meat extract, 5,0 g of peptone and 15,0 g of agar powder, put them in a flask and mix. Heat the flask in boiling water to dissolve the contents thoroughly. Use a 0,1 mol/l solution of sodium hydroxide to bring the pH to (6,8 ± 0,2) at 25 °C. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if nutrient agar is not used immediately, store it at 5 °C to 10 °C. Keep the medium temperature between 45 °C and 48 °C when mixing with a bacterial suspension.
Other culture media may be selected based on the requirements for the bacteria selected for the testing. Details of the selected media shall be included in the test report under culture conditions.
Prepared culture media may be stored at 5 °C – 10 °C for up to one month.
4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP)
For 1 000 ml of purified water, take 17,0 g of casein peptone, 3,0 g of soybean peptone, 5,0 g of sodium chloride, 2,5 g of phosphoric acid monopotassium dehydrogenate, 2,5 g of glucose and 1,0 g of lecithin, put them in a flask and dissolve them. Add 7,0 g of non-ionic surfactant and dissolve it. Use a solution of sodium hydroxide or hydrochloric acid to bring the pH to (7,0 ± 0,2) at 25 °C. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if SCDLP is not used immediately, store it at 5 °C to
10°C.
Prepared SCDLP solution may be stored at 5 °C – 10 °C for up to one month.
4.2.6 Phosphate buffer saline (PBS)
For 1 000 ml of purified water, take 34,0 g of potassium dihydrogen phosphate, put it in a flask and dissolve it thoroughly. Use a solution of sodium hydroxide to bring the pH to (7,0 ± 0,2) at 25 °C. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if PBS is not used immediately, store itat5°Cto 10°C.
Prepared PBS solution may be stored at 5 °C – 10 °C for up to one month.ISO 17721-1 pdf download.