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ISO TS 23034 pdf free download

ISO TS 23034-2021 pdf free download.Nanotechnologies一Method to estimate cellular uptake of carbon nanomaterials using optical absorption.
This document describes a near-infrared optical absorption method to estimate the in vitro cellular uptake of carbon nanomaterials including both internalized and/or tightly adhered to the cell membrane from liquid dispersions. This is a simple method to screen carbon nanomaterials uptake; additional analysis using a different technique can be required if quantification is desired.
2 Normative references
The following document is referenced in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods ISO/TS 80004-3, Nanocechnologies — Vocabulary — Part 3: Carbon nano-objects
3 Terms, definitions and abbreviated terms
For the purposes of this document, the following terms, definitions and abbreviations as well as the terms and definitions given in ISO/TS 80004-3 apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electropedia: available at http://www.electropcdia.org/
3.1 Terms and definitions
3.1.1 cellular uptake
internalization or association of a substance by a living cell
3.1.2 cell lysis
destruction or dissolution of cells with release of contents
3.1.3 absorbance
measure of the capacity of a substance to absorb light at a specified wavelength
3.2 Abbreviated terms
CNH carbon nanohorn
CNM carbon nanomaterial
CNT carbon nanotube
If A, km and t are known, then the concentration of a substance dissolved in the solution sample can be obtained. A is obtained by measurement, km is obtained by calibration using the known amount of the substance dissolved in the solution sample, and t is a fixed value determined by a cuvette optical pathlength for absorbance measurement.
4.2 Optical absorption of carbon nanomaterials
The optical absorption spectra of carbon nanomaterials in dispersion show a strong absorption peak in 300 nm to 200 nm (4 eV to 6 eV) that is attributed to the collective excitations of Tt electron systems (ir-plasmons).(2i This n-plasmon absorption peak can be also observed in most graphitic compounds.L1JJ The peak is superimposed on the featureless background extending to the vis-NIR and lR regions. The typical spectra of SWCNTs, MWCNTs, CNIIs, carbon blacks and graphene are shown in Figure 2. The broad non-resonant absorbance of MWCNTs, CNI-Is, carbon black, graphene and/or SWCNTs in vis-NIR region (e.g. 700 nm to 900 nm) is typical for most carbonaceous nanomaterials in samples.ISO TS 23034 pdf download.

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